Objective: E-cadherin is a tumor suppressor that expresses in normal epithelial tissues. Loss of it can be a cause of invasiveness in most carcinomas. Our study aims to investigate the effect of interval training on the E-cadherin gene in the breast tumors of mice.

Materials and Methods: 16 female Balb/c mice were divided into four groups. The cancer groups were tumorized with subcutaneous 4T1 cell line injection. An average intensity aerobic interval training protocol of 10 weeks was performed five sessions per week. E-cadherin gene expression was investigated using Real-time PCR method, and metastasis was evaluated with H&E staining in lung and liver tissues.

Results: Significant difference was found between the groups that participated in the training protocol before and after tumor induction and the non-trained group (p=0.031). Also, between the groups which participate in pre- and post-training protocol and the group that had only attended the preconditioning training protocol (p=0.042). The infiltration of cancer cells was visible in the lung and liver of the non-trained group.

Conclusions: According to the results, it can be claimed that aerobic interval exercise training may influence the key genes involved in the development of the metastasis process and even reverse this process.